Effects of CU300 or Natto yeast meal supplementation on production performance, egg quality and immunity in layers

Autor: Chun-Yi Liang, 梁純宜
Druh dokumentu: 學位論文 ; thesis
Popis: 103
Probiotic, prebiotics, and herbs are commonly used as feed additives to improve production performances of economic animals. In this study, the aim was to investigate the effect of CU300 (The mixture that consisted of γ-PGA fermentation product, fungal mycelia complex and herbs) or natto yeast meal (NYM; The product of protein mixture ingredients with two-stage fermentation by Bacillus subtilis and Saccharomyces cerevisiae) on growth, production performances, egg quality and immunity in layers. First part: The effect of different doses of CU300 on growth performance of developer. The γ-PGA fermentation product produced by Bacillus subtilis natto BS2, was analyzed on the physical and chemical properties of the product following extraction of γ-PGA. The extraction of γ-PGA is compared with pure γ-PGA which analyzed with thin layer chromatography (TLC) and SDS-PAGE. The result shows the single color dot and the same Rf value between the standard γ-PGA and the extraction of γ-PGA in TLC, and the molecular weight of extracted γ-PGA is greater than 130 kDa. The number of Bacillus colonies is approximately 9.58 log CFU/g in γ-PGA fermentation product and the concentration of γ-PGA is approximately 14.5% (w/w). Next, the γ-PGA fermentation, fungal mycelium mixture (Cordyceps militaris, Antrodia cinnamomea Ganoderma lucidum and Botryosphae riarhodina) and herbs, were mixed to produce CU300. One hundred and twenty 12-week old developers (Lohmann LB-LITE) were randomly assigned into five groups: basal diet with 0, 0.1, 0.2, 0.3% CU300 or 1% fish meal, and 6 replicates in each group. The result showed the growth performance of 0.1, 0.2 and 0.3% groups were better than the 0% group, and the 0.1% group was more commercial. Compared to 0%, the 0.1% group can improve feed intake, body weight, weight gain, and feed efficiency were 4.9%、3.0%、9.9% and 4.7%, and the performance of 0.1% group was similar to 1% fish meal group. Second part: The effect of different doses of CU300 on performance, egg quality, biochemical parameters in blood, and immunity of laying hens. Ninety-six 24-week old laying hens (Lohmann LB-LITE) were distributed into four groups: basal diet with 0, 0.1, 0.2 and 0.3% CU300 groups, 6 replicated. For performance, feed conversion rate was linearly improved with increasing supplemented doses during 24-45-week old (P
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