Study of Fabrication of composite Artificial Esophagus by Electrospinning

Autor: Lee, Chun-Hsien, 李俊賢
Rok vydání: 2014
Druh dokumentu: 學位論文 ; thesis
Popis: 103
This study is focused on preventing fall-off of the artificial esophagus, and applying electro-spinning fiber, carbon fiber and PLA/PCL solution shape into a tube as an artificial esophagus. First, observe the fiber structure and MTT assay of C2C12 cells with different ratio in solution, then find out suitable solution ratio for cell growing. Second, test cell culture cytotoxicity on carbon fiber, and design the scaffold sharp to improve the strength of suture. Then do tension strength test and continuities tension test. The result shows 8:2 Chloroform/DMF PCL solution with the smallest diameter and the best cellular activity. Carbon fiber is not conducive to growth of cell, but has no harm. Expanding tube effectively improve the strength of suture and also extend the time of fall-off.
Databáze: Networked Digital Library of Theses & Dissertations