Analysis of Interaction between FimB and FimE Using a Synthetic Circuit
| Autor: | Yu, Ying-Ying, 余盈穎 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 To investigate dynamic behaviors in biological networks, many biologists have devoted to establish customized biological systems in vitro or in silico. The task of exactly presenting the interactive behavior is challengeable owing to two reasons: 1) the extra regulatory factors involved in the system with high interaction cannot be totally considered; and 2) the interactive and dynamic behaviors results in great computational complexity on quantitative analysis. This study aims to design a reversible and regulatory switch for investigating complex interactions in fim systems in vitro. The orientation of the promoter within fimS which is flanked by two internal repeats, IRR and IRL, is decided by the ratio of FimB and FimE site-specific recombinases. FimE mediates recombination dominantly in the ON-to-OFF direction which expresses fimA, however, FimB with a slight bias for OFF-to-ON direction. We incorporated 12 biobricks (from iGEM) and two recombinases, FimE and FimB into a new biological system where several interactive relations were included. We monitored red and green fluorescence proteins whose expressions affected by FimB and FimE. The results showed the interactive dynamic of FimE and FimB which is mutual competition over time with given concentrations of anhydrotetracyclin (aTc). And the difference of the expression rates affected by FimB and FimE is distinct at different concentrations of aTc. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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