The study of progesterone receptor membrane component 1 in hepatocellular carcinoma
| Autor: | Jou-ChunLin, 林柔君 |
|---|---|
| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 To identify novel biomarkers associated with HCC, we have performed both MALDI-imaging and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on histology-proven HCC and adjacent non-tumor liver tissues. In-gel digestion with tandem mass spectrometry identified several interesting genes, including progesterone receptor membrane component 1 (PGRMC1) that belongs to membrane-associated progesterone receptor (MAPR) family. By using clinical specimen, we found that PGRMC1 is down-regulated in many HCC tissues which correlates with worse disease-free survival. In vitro, we used knockdown and overexpression approaches in the HCC cell lines to further evaluate the role of PGRMC1 in epithelial-mesenchymal transition (EMT), cancer stemness, cell proliferation, drug resistance, and autophagy. We found that PGRMC1 was neither associated with epithelial-mesenchymal transition (EMT) nor cancer stemness by western blot analysis. However, it seemed related with autophagy. Furthermore, PGRMC1 was related with cell proliferation and drug resistance through XTT assay. PGRMC1 overexpression decreased cell proliferation and reduced drug resistance in HCC cell lines. On the other hand, PGRMC1 knockdown increased cell proliferation and enhanced drug resistance in HCC cell lines. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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