Direct in situ butanol removal on the packed bed during continuous and immobilized Acetone-Butanol-Ethanol (ABE) fermentation
| Autor: | Yin-Rong Wang, 王胤融 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 In this study, a continuous Acetone-Butanol-Ethanol (ABE) fermentation system, cells of Clostridium acetobutylicum ATCC824 were immobilized by physical methods onto brick. The immobilization of cells can promote butanol productivity in the ABE continuous fermentation process. Physical methods were simple techniques that follow to maintain a higher cell concentration and grow the surface of carriers without toxic chemical. In addition to this, the bricks were used as the cell supports of which advantages were low cost and easily applied for immobilization. The other stage was consisted of two butanol separation process, liquid-liquid extraction and gas stripping. This novel integrated extraction-gas stripping hybrid process developed in our previous study has been shown a feasible and competitive in situ butanol removal technique for ABE fermentation where a record high solvent purity were obtained. To begin with, the objective solvent butanol was extracted by oleyl alcohol and refluxed to the immobilized bed. Then, gas stripping was performed simultaneously in the extraction phase. Finally, the extracted butanol was separated to the condensate from the continuous fermentation system. At a dilution rate of 1.00 h-1, where oleyl alcohol and feed flow ratio were 1, the glucose comsumption of 54 ± 2 g/L and the glucose utilization of 70 %, the butanol productivity and yield of 11.3 ± 1.1 gL-1h-1 and 0.21 ± 0.02 g-butanol/g-glucose, the ABE productivity and yield of 16.6 ± 1.8 gL-1h-1 and 0.31 ± 0.03 g-solvent/g-glucose were achieved with gas stripping flow rate of 6 LPM. The butanol concentration of 99-103 g/L in the condensate could be achieved. This study demonstrated that an enriched ABE fermentation can be achieved while a high purity of solvents can be simultaneously obtained for continuous fermentation. It should be concluded, from what had been said above, that gas stripping flow rate was selected to enhance butanol production efficiency. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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