The Blood Typing Chip for Blood Transfusion
| Autor: | Ching-Wei Huang, 黃經緯 |
|---|---|
| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 The purpose of blood typing is providing the blood of blood banking for the patients, surviving the input erythrocyte. The erythrocyte of patients will not destroy the composition of input blood causing hemolytic reaction. The blood bank must examine several routine inspection through the automatic blood inspection machine to insure the patient blood compatibility. But the automatic machine is expensive and take up more roomage. Cross matching usually uses to inspect the erythrocyte agglutination reaction of donators and donees. Recently, manual operation usually operates through the 96 wells and determines the class of erythrocyte agglutination by the naked eyes. But the 96 wells detection method is complex and time-consuming so that it cannot become automated test equipment. However, micro-fluidic chip can manipulate the micro volume such as reagents and specimen and carry on dilution, particle separation, mixture and reaction of the micro-fluidic transportation in the micro-channels. The purpose of the research is utilizing the MEMS technique to produce the clinical rapid blood typing chip which consists of micro-filter, micro-mixer, micro-channels, inspection area and reservoir wastes areas of complex chip. In the experiments, first all, the micro-channels of frontier chip was designed by separating the serum and blood and counted the serum collecting rate approximate 42.80% to 50.80%. The collected leukocyte on the front of micro-filter was approximate 8 to 9 cells, and on the erythrocyte collected champ was 0 cell. Secondly, the middle area of the chip was micro-mixer designed by mixing the reagents, combining the two colors dye to verify the mixture experiment. The straight micro-channels was adopted to be the control group simultaneously and analysis the mixture effects of two different micro-channels through the binary image. The result of the experiments is mixture micro-channels prior to the straights. Finally, the whole blood was injected in the chip for the blood typing examination. The agglutination class could be inspected by naked eyes on the blood type area in the end of the chip and counted the area of the erythrocyte agglutination by using the image program of the LabVIEW constructing the relations of the clinical and program. On the A type aspect, the pixels was 6601.5 and 24932.34 for the agglutination class +1 and +4 respectively. On the B type aspect, the pixels was 14955.75 and 22053.00 for the agglutination class +2 and +3 respectively. According to the data display of the image program, with the inhanced blood agglutination, the binary image also increased linearly. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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