Effects of burdock complex extract on Helicobacter pylori infection
| Autor: | Shu-yu Huang, 黃舒郁 |
|---|---|
| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 Helicobacter pylori infection is the major cause of chronic gastritis, gastric ulcer and gastric malignancy formation. In Taiwan, the prevalence of H. pylori approximately 55% most of H. pylori-positive patients is nonsymptomatic, but they had 10-20% risk to develop gastric diseases and 1-2% to develop gastric cancer which are unignored seriously problem. In clinically, the major treatment of H. pylori is triple or quadruple therapies which combine two antibiotics with either a bismuth compound or an antacid agents (proton pump inhibitor, PPI). However, the treatments cause several side effects, such as nausea, vomiting, diarrhea and abdominal pain that might lead poor medication adherence. Also, many studies suggest that some strains of H. pylori could mutate to resist antibiotic that reduced eradication rate. Recently, many studies investigated natural materials or food which could improve health, reduce disease symptom and even cure disease. In this study, burdock complex constituted by burdock (Arctium lappa), angelica (Angelica sinensis), gromwell (Lithospermum erythrorhizon) and sesame (Sesamum indicum) oil, which are commonly used in Chinese medicine. Many researches indicated all of four materials have antimicrobial, antioxidant and anti-inflammary effects. However, the inhibitory and preventive effects of these herbs on H. pylori remain unknown. The aim of this study is to investigate the inhibition of H. pylori infection by the supplement of burdock complex in clinical trial and determine whether burdock complex could inhibit H. pylori proliferation or adhesion in cell model. In clinical trial, we recruited H. pylori-positive subjects whose C13-urea breath test (UBT) value higher than 10‰, but they were nonsymptomatic. The H. pylori positive subjects (n = 36) were recruited and randomly divided into experimental and placebo groups. Burdock or placebo was taken for 8 weeks and 2 weeks of follow-up period after administration. The UBT value, antioxidant capacity, and inflammatory markers were analyzed and compared at the initial, 4th, 8th and 10th weeks. UBT value was significantly decreased in experimental group compared with base line, and no significant difference in placebo group. Moreover, as inflammatory markers, only tumor necrosis factor α (TNF-α) was decreased and had significant difference in 10th weeks. Also, antioxidant activity and total phenolics levels were increased after consuming 8 weeks of burdock complex. In cell model, the AGS cell was co-cultured with H. pylori then treated burdock complex extract, we found out the burdock complex extract could not inhibit H. pylori proliferation but inhibit adhesion of H. pylori to AGS cell. Burdock complex could improve H. pylori infection by inhibiting H. pylori adhesion on gastric epithelial cell and increase antioxidant capacity. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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