Inhibitory effect of skin-whitening agents on tyrosinase activity under varying pH conditions and the synergistic effect of skin-whitening agent mixtures
| Autor: | Ji-Yu Lin, 林紀妤 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 Skin whitening has invariably been a skin effect desired among Asian women. Overexpression and accumulation of melanin influence the overall aesthetics of a person and easily induce various types of skin pigmentation diseases. Tyrosinase is a key enzyme for melanin synthesis. Inhibiting the activity of this enzyme and accelerating the metabolism of melanocytic skin cells yield skin-whitening effects. In this study, α-arbutin, L-ascorbic acid, hydroquinone, kojic acid, magnesium ascorbyl phosphate, sodium ascorbyl phosphate, and tranexamic acid were used. The inhibitory effects that mixtures containing either two of the aforementioned substances have on tyrosinase activity were investigated under varying pH conditions ( pH=3~8 ). Subsequently, the effects of these mixtures on cytotoxicity and intracellular tyrosinase activity were determined. The experimental results showed that the samples most of the optimally inhibited the activity of mushroom tyrosinase under a pH condition of 3. Mixing kojic acid with hydroquinone exerted a synergistic effect; in addition, when 0.7% kojic acid was mixed with 0.3% hydroquinone, the optimal mixing effect under the same final concentration was achieved. For this mixture, the cytotoxicity test revealed that the toxicity of hydroquinone decreased with increasing proportion of kojic acid. The test results for intracellular tyrosinase activity showed that this aspects also decreased as the proportion of kojic acid was increased. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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