Preparation of Cationic PLGA Micelle Conjugated with Polyurethane Dendrimer for Delivery of DNA
| Autor: | Zen-Hung Chung, 鍾仁宏 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 Gene therapies using DNA and siRNA have been widely investigated in fundamental research. There are many methods to increase the transfection and gene expression including viral and non-viral vectors. Due to many drawbacks of viral vectors, non-viral carriers became another choice for gene therapy. Generally, the polymeric micelles were usually composed of amphiphilic block copolymers. The research was designed to use polyurethane dendrimers(PUAD) as a hydrophilic periphery. Because of the dendrimers with cationic characteristic, therefore, we conduct some test on the polymeric micelles to make sure that their potential to be a transfection vector. Biodegradable material poly(D,L-lactide-co-glycolide)(PLGA) was used as a inter hydrophobic core and reduced the toxicity of cationic PUAD. In this study, we used different generations of PUAD (G 2.0, 3.0) and PLGA to form amphiphilic block copolymers by dehydration reaction and their chemical structures were confirmed using FT-IR and NMR. The following tests were to evaluate the characteristics of PLGA-PUAD micelles: DLS to analyze its particle size and zeta-potential, electrophoresis, cytotoxicity, and transfection efficiency. It was found that the PUAD-PLGA can become a potential non-viral gene vectors based on the above results. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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