JAK-STAT is Hyper-Activated and Unusually Mediates the Loser Elimination in Myc-Induced Cell Competition
| Autor: | Chih-Ying Chiang, 江致瑩 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 Cell competition is a phenomenon, where the optimal cells (winner) eliminate and replace suboptimal cells (loser) in the same tissue. Cell competition is an evolutionarily conserved mechanism from Drosophila to mammals, and it is involved in organ size regulation and tumorigenesis. In most cases of cell competition, winner and loser cells are different in cell growth, proliferation, and protein translation. Myc and JAK-STAT signaling pathway are evolutionarily conserved in multicellular organisms and also critical in cell growth and development. When a cell is deprived of JAK-STAT or Myc, it becomes a loser eliminated by surrounding WT cell. A previous study indicated that Myc and JAK-STAT are independent in cell competition. Intriguingly, we found that in MycRi-induced cell competition, STAT transcription is activated and down-regulation of JAK-STAT components rescue the clone size. Here, we focus on the relationship between three JAK-STAT components (Domeless, Hop and STAT92E) and Myc. We use Flip-out-GAL4-UAS system to quantitate the degree of cell competition and represent significant data regarding the role of JAK-STAT and Myc in cell competition. Our results demonstrate that STAT activity is required for Myc-induced cell competition. Myc may modulate STAT signaling activity in cell competition. Taken together, we consider that STAT activity is harmful and modulated by Myc in Myc-induced cell competition. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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