Surface modified electrospun fibrous poly(3-hydroxybutyrate) membranes for tendon to bone healing
| Autor: | Hsueh-Wei Tseng, 曾學偉 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 The purpose of this study is to evaluate the properties of surface modified electrospun poly(3-hydroxybutyrate) (PHB) membranes for biomedical applications. The surface of electrospun fibrous PHB membranes was treated with hydrogen peroxide solution to create hydrophilic hydroxyl groups. Afterwards, dopamine (DA) was reacted with the hydroxyl groups by immersed the membranes in dopamine solution, and chitosan (CS) was then immobilized onto the membranes surface by using glutaraldehyde (GA) as a coupling agent to form covalent bonds between dopamine and chitosan. The growth factors (GFs) in platelet rich plasma (PRP) were then immobilized on the CS-grafted surface through the covalent binding of the peptides in GFs to the free NH2 groups in CS. The SEM images showed that the electrospun PHB membranes were composed of smooth and random-oriented fibers. The water-contact angle measurement indicated that the surface of membranes were wettable after surface modification. Via X-ray photoelectron spectroscopy (XPS), successful grafting of DA, CS and PRP on the membranes surface was demonstrated. The ninhydrin assay demonstrated the cross-linking degree of immobilized amount of TGF-β1 and PDFG-AB. The results of in vitro cell culture test of rabbit tenocytes showed that these functional membranes had good biocompatibility, as the cells showed good attachment and proliferation activity. A significant increase in collagen type I content and gene expression (e.g., Collagen I) in tenocytes was observed on the surface treated with the immobilized GFs peptides. Therefore, the modified electrospun PHB membranes showed great potential to be used in tendon to bone healing tissue engineering. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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