The effect of spirulina platensis on chronic tinnitus

Autor: Wu, Tzu-Ying, 吳姿穎
Rok vydání: 2014
Druh dokumentu: 學位論文 ; thesis
Popis: 102
Spirulina platensis has been proven to improve salicylate-induced tinnitus, but the effect on chronic tinnitus is still unclear. This study was aimed to examine the effects of Spirulina platensis on noise-induced chronic tinnitus and related modulators. Three-month-old male C57BL/6 mice were used in this experiment. The auditory brainstem response (ABR) of each mouse was detected, and then mice were trained by active avoidance test for two weeks. After exposed to noise (120dB, 3 hours), the mice were screened and those with tinnitus symptom were enrolled to the next diet intervention stage. Tinnitus mice were divided into control and 200 mg/Kg BW Spirulina platensis groups. Body weight, food intake and behavior performance were recorded for 80 days. After sacrificed, the inflammatory factors, including tumor necrosis factor-α (TNF-α), leukotriene-1β (IL-1β) and cyclooxygenase-2 (COX-2) mRNA expressions of temporal lobe, brain stem and the inner ear were examined. The regulation factors of tinnitus, including N-methyl-D-aspartate receptor 2B (NMDA2B receptor) and matrix metalloproteinase-7 (MMP-7) mRNA expressions were also analyzed. The results showed Spirulina platensis could reduce the false positive response, whereas there were no impact on the successful avoidance responses. Spirulina platensis significantly reduced the TNF-α, COX-2 mRNA expressions of the midbrain and brain stem tissues, and lowered TNF-α mRNA expression of inner ear. Spirulina platensis diet group showed higher IL-1β mRNA expression in the temporal lobe, midbrain and brainstem. In addition, Spirulina platensis effectively enhanced MMP-7 mRNA expression in temporal lobe, brain and inner ear. However, the expression of NMDA2B receptor did not differ between different groups. In summary, the supplementation of Spirulina platensis could retard the progress of noise-induced chronic tinnitus in C57BL/6 mice, which might due to up-regulate tinnitus related factor, and down-regulate the inflammatory factors.
Databáze: Networked Digital Library of Theses & Dissertations
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