In Vivo Rat Skin Penetration of Gold Nanoparticles : Geometric of Particle and Surface Modification
| Autor: | Ting-Cheng Tang, 唐婷鉦 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Nanoparticles are being used in multiple applications, ranging from biomedical and skin care products (e.g., sunscreen) through to industrial manufacturing processes (e.g., water purification). The increase in exposure has led to multiple reports on nanoparticle penetration. However, the correlation between gold nanoparticle shape and its penetration with chemical enhancers through the skin is poorly understood. Purpose of the present work is to study the effect of particle shape and surface modification on penetration of gold nanoparticles through rat skin. Besides flexible shape adjustment, the great popularity of gold nanoparticles originates also from their straightforward surface modification by formation of self-assembling monolayers (SAM) with thiol-containing functional group. Thus, we conjugated Poly(ethylene glycol) 2-mercaptoethyl ether acetic acid (SH-PEG-COOH) with the chemical enhancer (Oleylamine, OAm) and the PEG molecules attach to the gold nanoparticles surface through a sulfur-gold atom bond. The surface modification were characterized by UV-visible spectrophotometry, transmission electron microscopy, zeta potential analysis, and fourier transform infrared spectroscopy. Skin penetration of gold particles was observed by using confocal microscopy and transmission electron microscopy, the images show that rat skin revealed accumulation of gold nanoparticles in dermis of skin and the penetration amount of modification gold nanoparticles were higher than bare particles. As a result, the penetration of gold nanoparticle through skin can be enhanced by chemical penetration enhancement. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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