Effects of dexamethasone-encapsulated lipid nanoparticles in Raw 264.7 cells
| Autor: | Hui-Ling Chang, 張慧玲 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Dexamethasone is a synthetic glucocorticoid. On clinical application, dexamethasone with the properties of anti-inflammation could treat autoimmune diseases. It also could be chemotherapeutic drugs to treat leukemia. But long-term usages of dexamethasone often produce the side effects such as metabolism dysfunction, hypertension or dyslipidaemia. How to achieve the effective goal and reduce the dose and side effects is a problem. According to the reference, encapsulation of dexamethasone into nanoparticles could improve the effects on anti-inflammation and lipid nanoparticles possess the advantages of high biocompatible and low toxicity. In this study, dexamethasone was encapsulated into cholesterol-based nanoparticle GCC and the optimal ratio of GEC-cholesterol : cholesterol : dexamethasone was 1 : 1 : 1. The encapsulation efficiency (EE %) was measured by HPLC and the value was approximately 68 %. In the pro-inflammatory factors secretion experiment, the TNF-α and MCP-1 secretion of Raw 264.7 cells treated with LPS or zymosan showed similar results. Raw 264.7 cells treated with LPS followed by GCC did not reduce the TNF-α and MCP-1 secretion compared to LPS treatment but GCC_Dex reduced the TNF-α (approximately 20 %) and MCP-1 (approximately 30 %) secretion compared to dexamethasone treatment. Resemblance to LPS, Raw 264.7 cells treated with zymosan followed by GCC did not reduce the TNF-α and MCP-1 secretion compared to zymosan treatment alone but GCC_Dex approximately reduced the TNF-α (30 %) and MCP-1 (25 %) secretion, compared to dexamethasone treatment. These findings showed that encapsulation dexamethasone into lipid nanoparticles could reduce TNF-α and MCP-1 secretion. With chemical modification of nanoparticles in the future to prolong the circulation time in blood and drug release time will have the potential on long-term disease control with lower dosage. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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