Studies on Rapid Analytical Method for Determination of Tetracyclines Residues in Edible Animal Tissues
| Autor: | Hsueh, Min-Sheng, 薛敏生 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 A method for rapid analysis of tetracyclines residual veterinary drugs in edible animal tissues was developed. Ultrasound and dispersive solid phase extraction (d-SPE) were used instead of separation shaker, solid phase extraction (SPE) and rotary evaporator in sample preparation. The extraction process was developed by modifying the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) approach to provide faster and more convenient extraction. The samples were extracted by using acetonitrile, methanol and 0.25% acetic acid (3:1:3, v/v). Seven tetracyclines were determined by liquid chromatography tandem mass spectrometry (LC/MS/MS). Good linearity was observed from the calibration plots at 2.5-500 ng/ml. Recovery studies of analytes were performed at 50, 100 and 200 µg/kg spiked levels. Average recoveries ranged 72.2%~95.8% with relative standard deviation less than 10%. The limit of quantification was 5 µg/kg for muscle and 20 µg/kg for liver. Residues of tetracyclines (TCs) were detected from 12 chicken samples including 6 chicken, and 6 silky chicken collected from the supermarkets, sale stores, and traditional markets by the public health bureau from March, 2014 to April, 2014. The results showed that four chicken samples were found to contain oxytetracycline, five chicken samples were all found to contain doxycycline. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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