Effects of resveratrol on aberrant gene methylation and cell migration in nasopharyngeal carcinoma
| Autor: | Jia-cheng Lin, 林嘉程 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Resveratrol, a naturally dietary compound, has been reported that it affected many pathways to cause apoptosis or to inhibit cell proliferation in various cancers. But we were not sure about the effects of resveratrol on aberrant hypermethylation levels of tumor suppressor genes and cell motility in nasopharyngeal carcinoma (NPC). First, we treated four NPC cell lines, TW-04, TW-076, HK-1 and C666-1 with various concentrations of resveratrol. This resulted in significant decrease in cell viability when the concentration of resveratrol was equal or higher than 100 μM. On the other hand, resveratrol treatment didn’t cause cell death in normal cells, NP-69. Next, we studied RASSF1A, DAPK, RARβ2 and MGMT methylation statuses in NPC cell lines by methylation-specific PCR (MSP), and compared them with those in resveratrol treated NPC cell lines. The aberrant hypermethylation of RASSF1A, DAPK, RARβ2 and MGMT was detected in all NPC cell lines (TW-04, TW-076, HK-1 and C666-1). At least one of tumor suppressor genes could be partially demethylated after treatment with resveratrol. Four of these genes could be demethylated at different levels in NPC cell lines upon treatment of resveratrol. Moreover, we treated resveratrol to NPC cell lines and analyzed with Boyden’s chamber migration assay and wound-healing assay. We found that the cell migration abilities of resveratrol-treated TW-04, TW-076, HK-1 and C666-1 cells were decreased 37%, 78%, 62.5% and 86%, respectively. Our results implicated that resveratrol treatments reduced tumor suppressor genes’ aberrant hypermethylation levels in NPC, and might affect the regulation of cell motility in NPC. To sum up, our findings pointed out that resveratrol had potential to be developed as an anti-cancer drug and to prevent NPC metastasis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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