Biosensor for detection of asthma biomarker-ECP and anti-cancer drug-cisplatin
| Autor: | Wu, Li-Ping, 吳麗評 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Eosinophil cationic protein (ECP) was used as biomarker for asthma, which was been proved that bound with heparin and heparan sulfate existeded on cell membrane specifically. Based on the specific binding between ECP and heparin and heparan sulfate, gold nanostructured platform was simply formed on the working electrode through controlled electrodeposition, and then, the heparin was immobilized on the gold nanoparticles using the cysteamine as the linker. A electrochemical biosensor for detection of asthma biomarker was developed. other research of our lab improved the detection of ECP by immobilizing heparin on the Au@Fe3O4 NPs. This study further investigated the functional magnetic nanoparticles (Hep-Au@Fe3O4 NPs) in biological applications. We confirmed the functional magnetic nanoparticles are non-toxic and have potential to target drug development. The functional magnetic nanoparticles could be further applied into more extensively usages in the future. Cisplatin is commonly used in chemotherapy. But the side-effects it caused often makes patients discomfortable and even affects their liver and kidney function. Some studies have indicate that the binding between cisplatin and guanine will interfere the formation of DNA double-stranded structure. In this study, we used the telomere of human, a region of repetitive nucleotide sequences that contained a lot of quanine, combining with the character that performing larger fluorescence signal after binding to double-stranded DNA of SYBR ® Green.A simple strategy to sensitively detect cisplatin in human serum have developed. This method may have good prospects for drug research and development field. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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