Synthesis and characterization of the dual functional contrast agent CHC-[Gd(DOTA-NH2)]-FITC-Erb for magnetic resonance imaging
| Autor: | Li, Chia-Min, 李佳旻 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 In this study, the non-toxic and good biocompatibility of Carboxymethyl-hexanoyl chitosan (CHC) is used as a carrier, which can be utilized as the surface functional group to bond with [Gd(DOTA-NH2)], fluorophores and monoclonal antibody (Erbitux). The targeted biofunctional contrast agent CHC-[Gd(DOTA-NH2)]-FITC-Erb is synthesized and characterized for MR imaging and optical imaging. When conjucate CHC, fluorophores and monoclonal antibody, the relaxivity (r1) value of CHC-[Gd(DOTA-NH2)]-FITC-Erb is 15.4 ± 1.7 mM-1s-1 at 20MHz and 37.0 ± 0.1℃. This represents a 3-fold increase compared to [Gd(DOTA-NH2)]. Further in vitro MR-imaging analysis, CHC-[Gd(DOTA-NH2)]-FITC-Erb obtained negative signal enhancement is + 126.4％, better than those of [Gd(DOTA-NH2)] (+ 73.8％) and commercially available contrast agent Omniscan (+ 54.7％). Tumor cells (A431, SKBR-3 and Colo-205) were cultured with CHC-[Gd(DOTA-NH2)]-FITC-Erb. The results obtained from MR-imaging and optical imaging analysis indicate that the contrast agent CHC-[Gd(DOTA-NH2)]-FITC-Erb is the potential specific target for EGFR phenotype of tumor cells, espectially for A431. Our data shows that the successful synthesis of a specific targets of the image contrast agent CHC-[Gd(DOTA-NH2)]-FITC-Erb have not only the high biocompatibility and low toxicity, but also the image of both MRI and optical characteristics of the dual function. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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