Water-methanol extract from Euphorbia neriifolia L. inhibits viability of human hepatoma cells
| Autor: | Jun-Hua Lee, 李駿華 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Euphorbia neriifolia L. is an Euphorbiaceae succulents, native to northern India. It is widely used as a traditional folk prescription after incoming Taiwan: can be boiled as a tea for the treatment of malignant tumors. This study investigated the cytotoxic effects and active ingredients in of E. neriifolia tea. MTT assays for cell viability suggested that E. neriifolia extracts inhibit cell proliferation effect of liver cancer as a result of extraction conditions: extract from hot water extraction possessing cytotoxicity of HepG2 cells was significantly greater than the cold water extracts; the methanol-soluble portion of crude extract by hot water showed cytotoxic activity on hepatoma cells. This study verifies the traditional herbal medicinal effects, and its active ingredients were further analyzed by using column chromatography. It was found that, ingredients eluted with 90% dichloromethane and 10% methanol from the silica gel column have excellent inhibitory effect on human hepatoma cells and slightly inhibitory effect on HSC cells; however, preliminary tests on normal rat liver cells did not show significant toxicity. Purified ingredients from the SPE column illuminate strongly blue fluorescence under 365 nm UV. Determined by LC-MS, the main m/z of the peak further purified from the C-18 HPLC column with cytotoxicity is 631.93. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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