Affinity Biosensor of the Ratio of HbA1c to Hemoglobin Based on Impedance Measurement
| Autor: | Chang-WeiFan, 范正緯 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 In order to control and detect diabetes, some scholars and researchers had pointed out glycated hemoglobin (HbA1c) is one of the diagnostic assays to reflect the glucose level for long-term monitored. To improve the drawbacks of instruments for HbA1c detection, such as large, bulky and difficult to operate, a portable device is proposed to detect the ratio of HbA1c to Hb; with ring-shaped interdigital electrode based on impedance measurement. It is label-free, low sample consumption and no other reagent is required while the measurement process, because this is an on-chip biosensor. The ring-shaped interdigital electrode is first modified with self-assembled monolayer to immobilize HbA1c, then measure the impedance caused by protein through portable device, and calculate before-after impedance deviation through the electrical model. A theory of the impact of protein coverage to impedance deviation is proposed in this study. By separating the HbA1c in Hb on ten separation electrodes with microfluidic channel, and measure the impedance deviation for ten electrodes, finally, the area of protein covered can be obtained through the theory proposed in this study. The different ratio of HbA1c to Hb can be figured out by summing the area of protein covered on each separation electrodes. The 5% to 15% ratio of HbA1c to Hb are successfully detected through the portable device and intedigital electrode in this study. Because not only the drawbacks of large and bulky for commercial instruments are improved, and is also easy to determine the ratio of HbA1c to Hb with impedance measurement which is a simple way to analyse, it is close to the clinical needs extremely of this research. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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