Changes in systemic and udder innate defense functionality and udder remodeling of mastitis cows during the inflammation-resolution course of antibiotics treatment
| Autor: | Cheng-Ting Chen, 陳政廷 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Bovine mastitis continues to be the most economically important disease of dairy cattle and the costs include treatment, culling, death and decreased milk production. Mastitis is an inflammation of the mammary gland that often develops in response to intramammary bacterial infection. Staphylococcus aureus and Escherichia coli are among the most prevalent species of gram-positive and gram-negative bacteria, respectively, that induce clinical mastitis. E. coli is usually acute and often resolves spontaneously within a short period. In contrast, S. aureus mastitis often becomes chronic and may persist for the life of infected animals. The innate immune system of animal provides detection of the presence of foreign pathogens and mounts an inflammatory response. Prolonged inflammation often adversely and permanently impairs the productivity of mammary gland. The purposes of the current study were to monitor the changes in systemic and udder defense functionality and mammary gland remodeling during the inflammation-resolution course of antibiotic treated naturally acruired mastitis cows. Naturally acruired mastitis cows were confirmed by CMT and the antibiotic treatment was commenced the same day. Sampling was performed at four time points: the day of CMT confirmation but prior to antibiotic treatment and the second of the 3-day antibiotic treatment period (inflammation period), as wll as 1 day posterior to the 3-day antibiotic treatment period when CMT confirmed cure and 5 days posterior to the 3-day antibiotic treatment period whennormal milking was resumed (resolution period). Blood samples were collected from mastitis cows and the corresponding healthy cows (controls), while milk samples were collected from the infected udder and its contralateral uninfected udders. Overall, three mastitis cows and three control cows completed the whole sampling scheme. No difference was detected in blood total luekocyte count and total plasma protein content between mastitis and healthy cows, while milk somatic cell counts decreased during treatment in infected udders. Total milk protein levels were no difference in infected and uninfected udders except higher in one infected udder during inflammation. Phagocytosis activity of somatic cells was not different, while their respiratory burst activity was higher during inflammation then during resolution. Lower free radicals level in milk was found in infected than uninfected udders during inflammation. Higher levels of total immunoglobulins, BSA, and lactoferrins were found in milk of infected than uninfected udders during inflammation. Level of caseins in milk showed a reverse trend. MMP-9 is commonly used as a marker of inflammation, which is not found in normal milk, we reported higher level of MMP-9 in milk of inflamed udders and gradually decreased during antibiotic treatment except one cow was detected milk MMP-9 in uninfected udder, likely presence of subclinical mastitis. Taken together, following antibiotic treatment, phagocytosis activity of milk somatic cells, milk levels of BSA, lactoferrins, and MMP-9 declined gradually while milk casein level gradually increased. Milk MMP-9 level in mastitis udder closely reflected the physiological status of mastitis udders duing the inflammation-resolutionm course and, therefore, has the potential to serve as a real-time indicator of mastitis outcome. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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