The Internalization and Intracellular Transport of Exogenous Mitochondria in Fibroblast Cells
| Autor: | Wen-Yu Yeh, 葉文瑜 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Supportive therapies, rather than targeted therapies, are currently applied to the patients with genetically mitochondria-defective diseases. Previous studies showed that directly adding exogenous mitochondria to mitochondrial-defective cells can restore their respiratory and proliferative activities. The efficacy of mitochondria transfer can be improved by using cell penetrating peptide (CPP), which are known as carriers for exogenous macromolecules. CPPs bring the cargo into cells through cell-membrane penetration directly, but not through the passive endocytosis. Here, we found that adding biotin-TAT, a traditional CPP, into mitochondrial mixtures doubled the average size of mitochondria, determined by dynamic light scattering (DLS) analysis. The increased particle size was not diminished after sonication, suggesting that membrane fusion occurred between the outer membranes of mitochondria. We also demonstrated that applying biotin-TAT increased the efficiency of mitochondria delivery into cells and consequently promoted the restoration of the cell proliferation in oxidative-injured cells. This result suggests that phagocytosized mitochondria are not digested by endo-lysosomes and may be resorted to cytoplasma. Tracing the traffic of exogenous mitochondria in the targeted cells, we observed that only few mitochondria were colocalized with the EEA1+ early endosomes and LysoTracker-labeled endosomes. Whether exogenous mitochondria only transiently reside in the early endosomes requires further investigation. These preliminary results encourage us to further explore the detailed routes of exogenous mitochondria transport in the cells. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|