Biosensors based on graphene/glucose oxidase/nafion nanocompsite films
| Autor: | Hou-Kuan Li, 李侯寬 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 The preparation, characterization, and sensor application of the long- and short-chain graphene oxide (GO) derivatives prepared by the modified Hummers method were investigated in this study, also use AFM research the surface structures. The fabricated GO and reduced graphene oxide (RGO) used as a reinforcing material for conducting polymer were characterized by UV-Vis spectroscopy、FT-IR、XRD、Raman and Electrochemistry method. The RGO removed a large number of oxidation function group by reducing agent will become less hydrophilic and be difficult to disperse in solution. In the next part, RGO was used to prepare a biosensor electrode for the detection of glucose concentrations by direct electrochemistry of glucose oxidase (GOD). The morphology of the RGO/ GOD/ Nafion nanocomposite was characterized by atomic force microscopy. The root-mean-square roughness value of the film surface decreased significantly after GOD was immobilized in the RGO/Nafion nanocomposite film. The electrochemical performance of the RGO/ GOD/ Nafion modified electrode was investigated by cyclic voltammetry. Cyclic voltammetry measurements of the immobilized GOD showed a pair of well-defined and quasi-reversible redox peaks at the RGO-based electrode and provided evidence for a surface-controlled electrode process. Cyclic voltammetry results showed the biosensor to have good sensitivity of 11.02μA mM-1 cm-2 and linear concentration range of 0.9 to 6 mM (R2 = 0.995). The biosensor exhibits good reproducibility and anti-interference properties, and is suitable for application in the determination of glucose in synthetic serum. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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