Antityrosinase Mechanism of 36H: a Novel Tyrosinase Inhibitor
| Autor: | Li-Ching Lin, 林麗卿 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 36H is an active component that shows aniti-oxidantion and anti-tyrosinase activity. It was screened for antioxidant abilities using: 1,1-diphenyl-2-picrylhydrazul (DPPH), metal chelating power test and ferric reducing/antioxidant power (FRAP) examination. The result revealed that 36H had potential anti-oxidative capabilities. In addition, 36H was evaluated for tyrosinase inhibitory activities on in vitro mushroom, B16-F10 mouse melanoma cells and human melanocyte cells. Results showed that 36H owned significant inhibitory effects on the enzyme activities and no obviously decreased cell viability. Besides, through the quantitative real time polymerase chain reaction (QRT-PCR) and western blot, we found it could down regulate melanogenesis, melanosome mature and transport related RNA and protein, Tyrosinase, Pmel17, Myo5a, Mreg and so on. Therefore, 36H could be possibly developed to a new skin whitening agent |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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