Molecular mechanism of red mold rice extract prevents cardiac hypertrophy in H9c2 cardiomyoblast cells induced by angiotensin II
| Autor: | Chian-Pei Li, 李芊霈 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Monascus species used in East Asian countries to enhance food color and medicine effency. Many studies demonstrated that red mold rice can prevent cardiovascular disease, but the regulation mechanism of red mold rice in cardiac hypertrophy is unclear. In this study, to investigate the regulation mechanism of red mold rice extract (RMR-E) on cardiac hypertrophy in rat heart myoblast cell line (H9c2 cells) induce by angiotensin II (ANG II). We divide H9c2 cells into eight groups, including control, ANG II, positive control group (RMR-E and LOV only), MS only, ANG II combined RMR-E, ANG II combined MS and ANG II combined LOV. In this study, MTT assay were used to detect the effect of RMR-E, MS and LOV on cell viability in H9c2 cell. Result shown that the optimum concentration of RMR-E, ANG II, MS and LOV to experiment were 75 μg/mL, 1 μM, 1 μg/mL and 0.5 μg/mL, respectively. To investigate the effect of RMR-E on ANG II-induced cardiac hypertrophy in H9c2 cells were determined by immunohistochemistry, bradford assay and atrial natriuretic peptide (ANP) mRNA expression. Results shown that RMR-E (75 μg/mL) show significantly attenuate cardiac hypertrophy of ANG II-induced H9c2 cells. Further, according to ROS assay, NF-κB DNA binding assay and cytokines (TNF-α and IL-1β) mRNA expression. Results shown that RMR-E (75 μg/mL) significantly attenuate ROS generate, NF-κB activity and reduce cytokines mRNA expression in ANG II-induced H9c2 cells. In conclusion, RMR-E attenuated ANG II-induced H9c2 cells hypertrophy through supperession ROS production and NF-κB activation. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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