Inhibition of the Esptein-Barr Virus Lytic Reactivation By the Ethyl Acetate Fractions from Polygonum Cuspidatum
| Autor: | Yi-Jie Chen, 陳怡潔 |
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| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Epstein-Barr virus (EBV) is a human herpesvirus, which infects lymphoid and epithelial cells. EBV is associated with several malignant diseases such as Burkitt’s lymphoma and Nasopharyngeal carcinoma. Our earlier study found that the ethanolic extract of Polygonum cuspidatum roots inhibits the expression of EBV lytic proteins. The purpose of this study is to investigate further the anti-EBV effect of the ethyl acetate fraction from Polygonum cuspidatum. MTT assay was used to determine the viability of P3HR1 cells; immunoblot and flow cytometry analyses, the expression of EBV lytic proteins and phosphorylation-ERK; real-time quantitative PCR, the transcription of lytic genes and EBV DNA replication. Result showed that the ethyl acetate fraction F1, F3, F3a and major compounds, piceid and emodin inhibit the transcription of EBV immediated-early genes, BRLF1 and BZLF1 genes, and decrease the expression of lytic proteins, including Rta, Zta and EA-D, and EBV DNA replication. These results suggest that the ethyl acetate fraction from P. cuspidatum effectively inhibits the transcription of EB virus lytic genes, reduced lytic protein expression and decreased its DNA replication. The study shows that F1, F3, F3a and emodin inhibit the EBV lytic cycle. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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