Screening the lactic acid bacteria of the microflora and evaluation gastrointestinal physiological function
| Autor: | Yu-Wen Kao, 高于雯 |
|---|---|
| Rok vydání: | 2014 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 102 Lactobacillus and other microorganisms can form a stable eco-system in many environments and can stimulate the intestinal peristalsis, improve the intestinal flora, the beneficial to human health research shows high potential of lactic acid bacteria possess. Lactic acid bacteria and micro-organisms can produce viscosity extracellular polysaccharides and secreted outside the cell. Many studies have found that extracellular polysaccharide produced by lactic acid bacteria have anti-ulcer, regulate immune activity effects. Screening of polysaccharide content of lactic acid bacteria, and the herbal extract, and mushroom of freeze-dried powder solution in this study. Test of the extracellular polysaccharide content of the laboratory No. 1 to No. 90 and known lactic acid bacteria such as LGG, CMU995, B.longum. The result shows the top three extracellular polysaccharide content was LGG (1.51%), No. 57 (1.39%), No. 65 (1.26%) of lactic acid bacteria. The top three polysaccharide content of herbal aqueous extract was Citrus reticulata Blanco (10.1%), cortex Dictamni (6.95%), Phytolacca acinosa (5.48%). The top three of the freeze-dried powder of mushroom polysaccharide content for the solution was Trametes versicolor (21.5%), Ganoderma lucidum 10% concentrate (18.82%), Ganoderma lucidum (18.78%). In addition, to screen the lactic acid bacteria from human, tube feeding to mice and the change of intestinal bacteria was observed. Then select No.13,19,29, CMU995 to mice intestines trial (n = 6) for seven days, after assessment of the results. No. 13, CMU995 was selected to improve the human intestines trials, the study was a double-blind experiment divided into A, B, C (13, CMU995, the former two mix) three groups (n = 13). The results showed that 5 days after taking lactobacillus, the viable counts of E. coli bacteria in B and C group has declined; Enterobacteria in group B has declined ; Lactobacillus in group A and group B showed a upward trend; Clostridium perfringens was significantly lower trend in group A. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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