Sesamin inhibits proliferation of human breast cancer MCF-7 cells via induction of cell cycle arrest and apoptosis
| Autor: | An-Ci Siao, 蕭安淇 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Breast cancer is one of the most common cancers among women in the Taiwan. Although there are effective drugs for treating advanced stages of breast cancers, women eventually develop resistance. One of the approaches to control breast cancer is prevention through diet, which inhibits one or more neoplastic events and reduces cancer risk. Sesamin is one of the major components in sesame and has been widely studied and proved to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, we have used human breast cancer cell, MCF-7, as an in vitro model to assess the efficacy of sesamin as an anticancer agent. Sesamin with 1, 10 and 50 uM on MCF-7 cells dose-dependently causes cells apoptosis (TUNEL analysis) and death. Apoptosis of MCF-7 cells accompanied with caspase-3 activation. Flow Cytometry analysis suggested that MCF-7 cells treated with Sesamin accumulated during the sub-G1 phase of the cell cycle. Furthermore, our studies showed that sesamin increases p53, Bax, Caspase-3 and p-Chk2 expression. Taken together, our results show that sesamin modulates cell apoptotic signal pathways and inhibits MCF-7 cells growth. And this suggested that sesamin could be a dietary supplement for prevention of breast cancer. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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