A Single Channel Stereo Microscopy with Biprism

Autor: Che-chun Su, 蘇哲俊
Rok vydání: 2013
Druh dokumentu: 學位論文 ; thesis
Popis: 101
Different from traditional two-channel stereomicroscopes, a biprism is installed in the parallel optical path of an infinity optical system to successfully shooting the left and right image pair with a 10x single objective. The equivalent lens for the infinity optical system is designed with Zemax and preceded optical parameter analyses to discuss the conformity with the optical imaging quality. Such equivalent lens and the biprism are further analyzed the overall optical system structure with LightTools. The results optimize the biprism parameters for the experiment. The left and right image pair in the result is verified the object depth with anaglyph glasses and physiological factors, where the latter presents the object depth perception with negative parallax by eye adjustment and convergence. Nevertheless, with distinct physiological systems, different lens adjustment and color sensitivity could result in different depth perception. The experimental results are calculated the features point of the image pair with SIFT-RANGC and RANGC that the disparity appears 0.244mm, and the depth shows 1.05mm. SFD is further utilized for establishing the depth information and 3D image information of the image pair. A biprism is included in the infinity optical system to acquire images with two angles of view through the single objective so as to present the stereoscopic perception. Comparing with Confocal microscopes, which rebuild 3D image information with multilayer 2D image information, this study could largely reduce the precise equipment and the installation is easier. Moreover, it could acquire image pair simply through single channel, in comparison with traditional two-channel stereomicroscopes.
Databáze: Networked Digital Library of Theses & Dissertations