To investigate CPEB4 function in neurons using CPEB4 knockout mice
| Autor: | Ta-Jen Liu, 劉達人 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 CPEB4 is an RNA binding protein and belonged to the CPEB (cytoplasmic polyadenylation element binding protein) family. CPEB4 is widely expressed in many tissues, like brain, liver and lung. CPEB4 is known to play roles in regulating mitotic cell cycle and pancreatic cancer progression. To investigate functions of CPEB4, our lab generated CPEB4 knockout (KO) mice. Using the cortical neurons cultured from wild type and KO CPEB4 cortices, I found the spines of CPEB4 KO neurons are slightly longer than those of wild type neurons and NMDA receptor -mediated calcium influx is also elevated in the KO neurons. Such phenotypes imply that synaptic plasticity in the KO neurons may be altered. Although CPEB4 is expressed in hippocampus and amygdala, CPEB4 KO mice have normal learning and memory in spatial recognition and fear conditioning tests. A previous study showed CPEB4 might be involved in neuronal survival under ischemic stress. Therefore, I performed OGD (oxygen glucose deprivation) experiments and found WT and KO neurons showed similar viability after OGD stress. In contrast, knockdown of CPEB4 reduced neuronal viability after the OGD treatment. Therefore, I think CPEB4 is involved in neuronal survival upon ischemic stress. Some mechanisms may developmentally compensate the CPEB4's function in the KO neurons. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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