Morphology Analysis of Suspension co-culture of Hs68 & HaCaT Cells
| Autor: | Chih-Hao Chao, 趙志豪 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 We developed a new co-culture method mainly in co-culturing cells in suspension. Unlike the traditional method, cells cultured by this method will aggregate to a sphere and form a 3D structure, which compared to other systems like the hanging drop method and the hydrogel method, our system is simple, easy and good in long term culture. Our research used fibroblast and keratinocyte in co-culture, and we adopted Hs68 cell line from fibroblast and HaCaT cell line from keratinocyte. These cells are both from mature male foreskin, and the interaction existing between the two cells have been confirmed. We used the shared characteristics of Hs68 and HaCaT, that both will suspend in chitosan coated plate, to perform a suspension co-culture method. In this system, we can easily observe how each cells aggregate in time-lapse photography. This paper also discussed the aggregation process in suspension co-culture system, which was analyzed by a simple simulation. We’ve got a first step results in hands-on practice at present. Academically, we also adopted the theory about cadherin to explain our model design, thus we conducted some experiments in measuring cell-cell cohesion force to support our model. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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