Enhancement of Taxol effectiveness on HeLa cells by Selected broad Bandwidth infrared radiation (IR)
| Autor: | Pei-Shen Huang, 黃佩慎 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Cervical cancer is the third most common cancer in women, with an estimated 530 000 new cases in 2008. More than 85% of the global burden occurs in developing countries, where it accounts for 13% of all female cancers. High-risk regions are Eastern and Western Africa, Southern Africa, South-Central Asia, South America and Middle Africa. Rates are lowest in Western Asia, Northern America and Australia/New Zealand. Cervical cancer remains the most common cancer in women only in Eastern Africa, South-Central Asia and Melanesia. Overall, the mortality: incidence ratio is 52%, and cervical cancer is responsible for 275 000 deaths in 2008, about 88% of which occur in developing countries: 53 000 in Africa, 31 700 in Latin America and the Caribbean, and 159 800 in Asia. Cervical cancer in Taiwan is still ranking primary in female cancer. About 6,000 people each year were diagnosed with new cases (including carcinoma in situ and invasive carcinoma), and nearly 900 people each year died of cervical cancer. Modern treatment of cervical cancer, the early stage patients accept radical hysterectomy, and there will be lots of systemic side effects such as difficulty in urination and defecation; the late stage patients can’t accept surgery, will receive chemotherapy plus radiation therapy. Chemotherapy combined with radiotherapy has been effective in some degrees of treating cervical cancer; however systemic side effects should not be underestimated. In previous study showed that persistent chronic infection with human papilloma virus (HPV) causes cervical intraepithelial lesions, further progress turned to cervical cancer in few years later. Simple HPV infection or mild cervical intraepithelial lesion treatment, recommended primary care with regular pap smear, colposcopy or biopsy when it is indicated. If the Infrared (IR) light irradiation can postpone the progression of the disease and induced HeLa cells apoptosis, IR may help in the treatment of cervical intraepithelial lesions and cervical cancer. The position of cervix is suitable for the use of photodynamic therapy combined with local chemical or radiation therapy due to its surface condition and the dark environment. Patients in Taiwan still not accept photodynamic therapy widely, because of the costs of the photosensitizer and laser light source. The main objective of this study, laser light is replaced by selected broad bandwidth infrared radiation, and photosensitizer is replaced by Paclitaxel. If the drug effect increased on HeLa cells irradiated by selected broad bandwidth infrared radiation, it changed the growth patterns of HeLa cells, and reduced cancer cell proliferation and progress. In recent years, our group demonstrated tri-layers Au/SiO2/Au waveguide thermal emitter (WTE) as the selected broad bandwidth IR light source. The cavity mode resonance is generated in the SiO2 layer between two Au films. It can be applied in gas sensing, life science research and biomedical engineering. We apply the same miniaturization infrared light source with cooling system, placed in a growth chamber with a stable environment (5% CO2，37.0±0.5℃) in our study.The first phase of the experiment, the human cervical cancer cell line HeLa cells firstly irradiated by selected broad bandwidth 3-5μm IR radiation without drugs. The second phase of the experiment, we choose Paclitaxel to study the effectiveness on HeLa cells with selected broad bandwidth IR radiation.Preliminary reports have found that IR can enhance the therapeutic effect of Paclitaxel. So we will continue to clarify the mechanism if IR may regulate mitochondrial membrane potential and produce a large amount of reactive oxygen (ROS) to enhance the effectiveness of Paclitaxel treatment. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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