Characterization of Oral Precancerous Lesions Based on Higher Harmonic Generation Microscopy
| Autor: | Chen-Yu Lin, 林宸羽 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Oral cancer is the fourth most common cause of male cancer death in Taiwan and ranks first among the cancers that affect the young male population. About 5,400 new oral cancer cases are reported in Taiwan each year. However, the five-year survival rates of oral cancer patients are still low. The only way to address this problem is early diagnosis. In the oral cancer diagnosis, surgical biopsy followed by pathological exam is the standard operating procedure. But, surgical biopsy is always accompanied by pain, bleeding, trauma, and spreading of cancer cells. Thus, developing a non-invasive diagnosis tool is relatively important. In this research, we used a noninvasive harmonic generation microscope to observe the ex vivo oral mucosa of 23 precancerous specimens, including leukoplakia and erythroplakia. In all of these specimens, there were 4 specimens excluded in our analysis due to the fact that we could not obtain the whole epithelium imaging of these samples. According to pathological diagnosis results, we separated 19 samples to carcinoma in situ, severe dysplasia, moderate dysplasia, mild dysplasia, and hyperplasia. We compared the five groups with their H&E stained slides. Moreover, based on the histopathological diagnosis criteria, we further analyzed the cellular density at one-third and two-third of epithelium to quantify the difference between the oral dysplasia lesions. In this analysis, we grouped the carcinoma in situ and severe dysplasia as severe dysplasia. The cellular density of severe dysplasia lesions had a significant increase comparing with other lesions at one third of oral epithelium (P value |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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