The Application of RNA Interference as an Anti Nervous Necrosis Virus Mechanism in Epinephelus sp.
| Autor: | Wei-Siang Chen, 陳薇向 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Viral infections have been increasingly reported in cultured marine fish. Among these infections, viral encephalopathy and retinopathy (VER), caused by betanodaviruses (piscine nodavirus), is one of the most devastating, occurring in a variety of marine fish all over the world. Histological examination of tissues from the central nervous system and the retina often reveals areas of conspicuous tissue vacuolation and necrosis. Their genome consists of two molecules of messenger sense RNA. RNA1 is approximately 3.1 kb in size and carries the gene that encodes for an RNA-dependent RNA polymerase referred to as protein A; RNA2 is approximately 1.4 kb in size and contains the open reading frame that encodes the capsid protein. NNV infected fishes will result in a large number of fish deaths after 8 – 35 days post-hatching. There is a defect by using antigen and antibody on the early stage of grouper’s adaptive immune mechanism as it may cause the grouper adaptive immune may not fully established in this stage. In order to protect the early stage of grouper, another mechanism must be applied to against NNV. RNA interference (RNAi) including two interfering mechanism: microRNA (miRNA) and small interfering RNA (siRNA); this is known as gene silencing which is mainly interfere the expression of protein in order to inhibit the expression of gene. In present, this mechanism was found in many eukaryotes and this RNAi mechanism is fully established after hatching. In this study, we applied RNAi technique to anti-NNV invasion and infection, increase the survival rate of groupers; in the meantime, we examined the relationship of RNAi and virus replication. Besides, we utilized different encapsulating materials and different combination of siRNA and the unique liposome-feeding system for the delivery of siRNA. From the experimental results, we found that siRNA can inhibit the replication of NNV in groupers. The differential expression of siRNA and virus particles can be observed during larvae rearing. We can conclude that the siRNA not only process a preventive ability but also treatment of virus infection. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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