Role of collagen matrix on HDGF of hepatocytes
| Autor: | Te-Wu Chou, 周德武 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Hepatoma-derived growth factor (HDGF) is a nuclear growth factor that is involved in the development of liver fibrosis. HDGF regulated the TGF-β expression while hepatocyte exposed to collagen matrix in vitro. Besides, HDGF gene delivery in mice would enhance the collagen matrix deposition in liver. However, the regulatory effect of collagen matrix on HDGF expression is remaining to be explored. The aim of this study is to investigate the effect of collagen matrix on HDGF expression of hepatocytes in the early stage of hepatic fibrogenesis. It was found that collagen matrix potently inhibited the proliferation of hepatocytes via blocking the G0/G1 phase of cell cycle. Collagen matrix up-regulated the HDGF and nucleolin expression and secretion levels of clone-9 and HepG2 cells. Moreover, collagen matrix also induced the phosphoinositide 3-kinase (PI3K)/Akt pathway. Ad-HDGF RNAi gene delivery would attenuate the collagen matrix induced HDGF, nucleolin and PI3K/Akt pathway up-regulation. In conclusion, we showed that collagen matrix up-regulated the HDGF expression and regulated PI3K/Akt pathway via HDGF expression to promote the pro-survival activity of hepatocytes and hepatoma cells. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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