Application of waste mushroom sawdust amended with Trichoderma koningii to control tree brown root rot caused by Phellinus noxius
| Autor: | Wei-Cheng Hsu, 徐偉城 |
|---|---|
| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Phellinus noxius is major wood decay fungi causing decline of many species tree in Taiwan. This research focuses on utilization of waste mushroom sawdust amended with Trichoderma antagonists to experiment the control efficiency of tree brown root rot caused by Phellinus noxius. 35 isolates of Trichoderma were isolated from soils and dead materials of plants at different areas in Pingtung. With cellophane antagonism test and dual culture, 3 isolates of Trichoderma. were selected based on the inhibitory effect of antagonism on mycelial growth of Phellinus noxius. Dual culture on slide between TAS、TDS and TFS isolates of Trichoderma and Phellinus noxius showed that these three isolates could degrade the mycelium of Phellinus noxius. By coling and penetzation all of three isolates were identified as species of Hypocera koningii (amamorph: Trichoderma koningii) by comparison of the sequence of ribosomal DNA. Potato dextrose ager (PDA) amended with individual fungicide was used to evaluate effective fungicides on suppression of Phellinus noxius. Among the 6 fungicides tested against Phellinus noxius, 45% difenoconazole propiconazde EC and 25% propiconazde EC were most effective in completely inhibiting the mycelial growth at the active ingredient (ai) dosage of 10 ppm. In composting process of waste sawdust, through the monitory of temperature, pH value and electronic conductivity of composted sawedust and Trichoderma cultivate test, the available sawdust was harvested and ultilized at 4 days after composting fermentation for Trichoderma culture. Effect of pH value and water content of sawdust culture substrate on mycelia growth of TAS、TDS、and TFS were experimented in large glass tube culture.The Trichoderma mycelia grow well at 5 and 7% of CaCO3 in sawdust, and was better tham than that of other CaCO3 content at 1and 3% Trichoderma grow its mycelium better at 70% water content of sawdust substrate than that of any other water content at 50, 60 and 80% rspectively. The study was conducted to determine survival of loquat in soil amended with different amount of waste mushroom sawdust. Results showed that soil amended with waste mushroom sawdust in 2:1(w/w) or 3:1(w/w) was effective growth of loguat in all the plants after treating for 1 month. When waste mushroom sawdust which had been grown T. koningii TAS, TDS, and TFS isolate was amended to diseased soil, the results showed that soil supplemented with the waste mushroom sawdust amended with Trichoderma koningii and drenched synthetic fungicide,were more excellent in disease control with no infected loquat occurred in all the plants after treating for 35 days. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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