Studies on the Anti-oxidant Activity of Flowers and Seeds from Aquilaria sinensis Grown in Taiwan
Autor: | Chia-Ting Lin, 林家鼎 |
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Druh dokumentu: | 學位論文 ; thesis |
Popis: | 101 Antioxidant activity of inquiry flowers and seeds from Taiwan artificial Aquilaria sinensis in this study.A capsule of Aquilaria sinensis holds one or two seed(s) in deep red color like chinese red bean. with average weight of 100.6 mg, each seed consists of seed coat and kernel, the percentage of which is 42.9%,57.7% respectively and 61.14% oil content of kernel。The seed contains various fatty acids: 54.70% of oleic acid (C18:1), 8.62% palmitic acid (C16:0), and 26.06% of other 20 fatty acids with aliphatic tails longer than 20 carbons.The DPPH inhibition rates of butanol soluble fraction and water soluble fraction of methanol extracts of Aquilaria sinensis blossom are 89.02 % and 85.88 % respectively, which are very close to that of commercially-used antioxidant - catechin, whose DPPH inhibition rate is 86.33 %, indicating strong capability of DPPH free radical scavenging. The water soluble fraction of blossom, seed coat and kernel of Aquilaria sinensis has stronger metal chelating ability than the butanol soluble fraction of them, while the ethyl acetate soluble fraction of them shows the weakest or even no metal chelating ability. The reducing power of the three soluble fractions of blossom is higher than that of seed coat and kernel of Aquilaria sinensis. Among the three soluble fractions of blossom and seed coat, butanol soluble fraction exhibits the highest reducing power respectively. General antioxidant ability of the three soluble fractions of blossom in concentration below 1mg/mL was evaluated. Strong antioxidant activity (30.30 μM of Trolox) was found in butanol soluble fraction, whose content of total phenolic compound is the highest among the three soluble fractions and even higher than that of rose. Compounds separated from Butanol soluble fraction of methanol extract of blossom include 5-hydroxy-7, 4’-dimethoxyflavone, 5,3’-dihydroxy-7, 4’-dimethoxyflavone and kaempferol-3-O-rhamnoside, which are the natural substances first discovered in blossom of Aquilaria sinensis grown in Taiwan as forestation species. |
Databáze: | Networked Digital Library of Theses & Dissertations |
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