Human Gingival Fibroblasts Cultured On Chitosan Film Crosslinked By Glutaraldehyde Could Inhibit Bacterial Invasion And IL-8 Relative Inflammatory Responses
| Autor: | Yu-Che Lin, 林郁哲 |
|---|---|
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Chitosan, which is a nature polymer, has been fabricated into various forms such as antibiotic additives in bone cements, micro/nano particles or reservoir devices for drug delivery and membranes or matrices for tissue engineering. However, there is no research indicating chitosan anti-bacterial abilities after cell culture. In this study, we have prepared chitosan liquids, particles and films by glutaraldehyde crosslinking reaction to study their effect on bacterial killing and the prevention of bacterial invasion on human gingival fibroblasts (HGF). The results have shown that all chitosan formulations had anti-bacterial abilities on E. coli and S. mutans. In comparison with chitosan particles, chitosan films and liquids have demonstrated higher antibacterial ability and no bacterial invasion into HGF. Interestingly, chitosan films crosslinked by glutaraldehyde present hydrophobic surface and which could not support cell adhesion. Therefore, various proteins were added onto chitosan films for modifying their hydrophobicities and then cell adhesive abilities. In bacterial invasion study, HGF cultured on chitosan films could inhibit bacterial invasion and gene expressions of inflammation markers such as IL-6, IL-8, ICAM and VCAM and COX-2 gene. Based on these results, we conclude that chitosan films could suppress inflammation responses on COX-2 pathway and prevent the adhesion and gathering of immune cells through the inhibition of IL-8, ICAM and VCAM expressions. Chitosan films crosslinked by glutaraldehyde showed good cell attachment and proliferation through protein coating and can inhibit bacterial invasion and further immune responses. Therefore, chitosan films crosslinked by glutaraldehyde could be potential in tooth and bone biomaterial applications. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|