The Application of Curcumin-loaded Liposomes on Osteoporosis Treatment
| Autor: | Yu-Han Su, 蘇鈺涵 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Curcumin (diferuloylmethane; Cur) is a non-water-soluble polyphenol compound that has anti-inflammatory potential. Recent studies found that Cur can reduce the osteoclastogenic activity for preventing bone loss. Osteoporosis is a skeletal disease due to an imbalance between bone resorption and bone formation. Osteoprotegerin (OPG) and RANKL have been shown to play important roles in bone remodeling. To increase cellular uptake of Cur, we used soybean phosphatidylcholine to encapsulate Cur for liposome formation. In this study, Cur-loaded liposomes have been characterized in particle size, encapsulation efficiency, liposome stability and cellular uptake. To study the effect of Cur-loaded liposomes in vitro, lipopolysaccharide and RANKL were added to induce osteoclastogenesis in rat macrophage (RAW264.7) and 50 μg/ml ascorbic acid and 10 mM β-glycerol phosphate were added to stimulate mouse osteoblast-like cells (7F2) differentiation. Besides, we used IL-1β to induce 7F2 cell inflammation to investigate the anti-inflammatory activities of Cur-loaded liposomes. The results show that there are about 70% entrapment efficiency of Cur in liposomes and particle sizes are stable after liposome formation. Cur-loaded liposomes can inhibit macrophage inflammation and differential activities. In comparison with Cur only, Cur-loaded liposomes have no significant cytotoxicity and can remain the osteoblast differential functions. With IL-1β stimulation, Cur-loaded liposomes can successfully down-regulate the expression of inflammation markers on osteoblasts and showed high Osteoprotegerin (OPG)/RANKL ratio to prevent osteoclastogenesis. In this study, we observed that Cur can be encapsulated in liposomes successfully and which can reduce osteoclast activity and maintain osteoblast functions. Therefore, Cur-loaded liposomes can provide a potential application in osteoporosis treatment. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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