Effect of single nucleotide polymorphisms in HSP70 and avUCP genes on semen quality traits during chronic heat stress and production traits under normal environmental temperature in chickens
| Autor: | Lin-Liang Peng, 彭麟量 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Heat stress causes major economical loss of poultry industry in tropical/subtropical area. Heat stress can result in high mortality, low growth performance, low egg production and reproduction disorders in roosters or hens. Heat-shock protein 70 (HSP70) is a molecular chaperone that can help cells to overcome heat stress. Avian uncoupling protein (avUCP) is known to play a role in regulating reactive oxygen species production in mitochondria. HSP70 and avUCP may be potential markers for thermotolerance in chickens. The purposes of this study were to investigate the effect of single nucleotide polymorphisms (SNPs) in HSP70 and avUCP on semen quality traits of roosters under chronic heat stress and on production related traits under normal environmental temperature. Experiment 1 used 55 31-week-old F2 reciprocal crossbred chickens between Rhode Island Red selected for low residual feed consumption and L2 strain Taiwan country chicken. Roosters received four weeks of 32°C, 55% relative humidity chronic heat stress and the semen quality traits were evaluated weekly. The SNPs of HSP70 and avUCP were analyzed by PCR-RFLP. The result indicated that CG genotype in HSP70-C276G had higher sperm concentration than GG genotype after one week of heat stress (P T in avUCP gene did not show polymorphism in this study. In XR female, GG genotype of HSP70-C276G had higher 8 wk body weight than CG genotype. There were no significant genotypic effects on XL male, XR male and XR female. In XL strain male, the CG genotype had better performance than GG genotype in body weight gain between 4 to 8 wk (P |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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