Functional characterizations of SERRATE-interacting proteins in Arabidopsis
| Autor: | Heng-Yi Huang, 黃恆毅 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Epigenetics is the study of changes in gene expression or cellular phenotype caused by mechanisms such as DNA methylation, chromatin remodeling and histone modifications, etc. Studies showed that specific histone modifications promote plant response to environmental stress. In Arabidopsis, ATX1 (ARABIDOPSIS HOMOLOG of TRITHORAX 1) and GTE12 (General Transcription Factor Group E 12) are able to recognize histone tail and then regulate gene expression. ATX1 has a histone methyltransferase activity, which is able to enhance gene expresion by H3K4me3 modification. It has been reported that ATX1 can regulate NCED3 to increase abscisic Acid (ABA) biosynthesis. ABA accumulation could promote stomatal closure to decrease transpiration during dehydration. SE (SERRATE) is a key regulator in miRNA processing. Using yeast two-hybrid screening and co-localization analysis, we showed interaction between ATX1 and SE. GTE12 has a bromodomain protein which is able to recognize acetylation site on histone tail and regulate genes expression. Previously, we found that the C-terminal domain of GTE12 can interact with SE by yeast two-hybrid screening. Here, RNA-seq analysis show that gte12-3 mutant can repress the gene expression of CYP707A3, which encoded a 8''-hydroxylase for ABA degradation during dehydration and rehydration. Taken togather, these results suggest that the interaction between SE and ATX1 or SE and GTE12 maybe required for epigenetic regulation of ABA response. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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