Endophytic bacterial communities of maize under different fertilization
| Autor: | Qiao-Zhen Wang, 王巧貞 |
|---|---|
| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Agricultural management and fertilizer application are known to affect the soil microbial community structure and diversity. Endophytes gain entrance into plant parts mainly through the root system. In addition, fertilization may influence soil microbial community and structure of endophytic bacteria. The purpose of this study was to investigate the structure of the root endophytic community in maize under different fertilizer treatments. PCR-DGGE analysis using 16S rDNA primers was used to elucidate the structure of the endophytic bacterial communities and nifH primer demonstrated diazotrophic bacterial species diversity in maize root. Functional diversity was measured by the carbon source utilization (Biolog) and nitrogenase activity (acetylene reduction assay) under different fertilization treatments. Rhizospheric microbial carbon utilization was also measured and compared with endophytic community. The results showed that maize root endophytic community structure was affected by chemical fertilizers, compost and green manure revealed by PCR-DGGE and Biolog analyses. In PCR-DGGE, the bacterial diversity of the manure compost treatment was higher than that of the chemical fertilizer; however, in Biolog tests, the diversity of the chemical fertilizer treatment was higher compared to the manure compost, Showing that the bacterial diversity tested by 16S rRNA gene and carbon utilization methods had interesting meaning. The diversity tested by Biolog was difference between soil and root. In soil, the carbon utilization diversity of the chemical fertilizer treatment was the highest, however, the diversity of the chemical fertilizer was the highest in root. The diazotrophic bacterial community structure in maize root tested by PCR-DGGE under the different fertilizers was not significantly different. However, the nitrogenase activity in maize roots was related to inorganic nitrogen content in soil. Endophytic bacteria may be used as biofertilizers with chemical or organic fertilizer. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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