Microfluidic Design for 3D Multiple Sampling Dispenser
| Autor: | Lai, Chien-Yuan, 賴建元 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 In this study, the microfluidic emulsion chips were manufactured using micro-electro-mechanical system (MEMS) technique. The theme of study followed the senior's micro multiple quantitative samplers[36] with back-end of the two-dimensional hydrodynamic focusing structure to produce a two-step emulsification biphasic emulsion droplets; The research is shape design of micro-channel. Using microfluidic channel, micro-pumps, micro-valves and nozzles with different diameters design applications to make quantitative droplet nozzle with micro-pump pressure control to determine the size of the droplets. Microfluidic channel can enter different sample, and sample between the sample does not mutual interfere. By connection hydrodynamic focusing structure, double emulsion droplets can be generated. This emulsion droplets integrated microfluidic chip, micro-pumps and micro-valves and other components, and similar T-shaped channel microfluidic emulsion technology with dynamic focusing structure to produce double emulsion droplets. By controlling the frequency controller and solenoid valve ON / OFF switch of the clock, pressure is the primary power source to drive the micro-pump and micro-valve. Micro-pump actuation is first taken to store fluid sample in the body cavity. After film compression, the sample out through the nozzle drops bottom flow channel, form an oil-in-water (W/O) emulsion droplets. Then, double emulsion droplets (W/O/W) were formed by using the hydrodynamic focusing structure at intersection of the oil phase and the external water phase. The emulsion droplets with different diameters were produced by change water phase and oil phase velocity. The chip in this study is to combine SU-8 negative photoresist in the process of photolithography to produce circular micro-channel mould. This mold can then be used to replicate circular micro channel with PDMS. Since PDMS is a soft material, there will be a crack on structure while demolding. However, the crack can be recovered during the process of thermal bonding of PDMS components, for PDMS molecules would permeability into the crack. Therefore we can reproduce microfluidic channels by PDMS demolding process. We successfully fabricate emulsification chips with hydrodynamic focusing structure to produce double emulsion droplets. These devices have shown great promise in various applications including emulsification. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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