Analysis of the enhancement factors in the SERS spectra of amino acids in silver colloids
| Autor: | Chih-Hsuan Chen, 陳志軒 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 This study used the Raman spectrometer to observe a variety of amino acid molecules (cysteine, phenylalanine, tyrosine, tryptophan) and thymine adsorbed on the surface of the silver colloids to enhance Raman scattering effect. When the amino acid molecules are mixed with silver colloids, different functional groups of the molecules will cause different orientations of the amino acid molecules when adsorbed on the surface of silver colloids, leading different Raman scattering intensity. In order to determine the functional group that causes the adsorption orientation difference, this study added aggregating agents and adjusted the pH of the solution in the experiments to investigate the adsorption of amino acid molecules on the surface of silver colloids. Calculation of the Raman enhancement factor showed that the addition of aggregating agents, such as potassium fluoride and potassium phosphate, can achieve the best enhancement of amino acid molecule surface Raman scattering effect. When the pH is in the range from 6.7 to 10, amino acid molecular mapping signals have an increased enhancement effect. In this pH range, the addition of potassium phosphate can achieve the best enhancement of surface Raman scattering effect. All experimental evidences showed that the particle size of the anionic aggregating agent, the charge number, and the pH of the solution are closely related to the enhancement of the surface Raman scattering effect. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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