Pathological and Biological studies of Eimeria flavescens
| Autor: | Wang, Ting-Chen, 王婷蓁 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 This thesis examines the pathological, biological studies and use molecular identification for Eimeria flavescens. Rabbit coccidiosis caused by Eimeria parasites. The Eimeria of rabbits is colonizing in the intestinal tract and one species infecting in the biliary ducts of the liver. Differentiation of Eimeria species in rabbits has been classically performed using a set of biological features such as oocyst size and morphology, prepatent period, site of colonization, amongst other aspects. In the molecular identification, we use to identify coccidium species. In the pathological study, a total of 10 coccidia-free rabbits were inoculated orally with approximately 1×104sporulated oocysts of the pure strain of Eimeria flavescens. Experimental animals were sacrificed at 1, 2, 3, 4, 5, 6, 7, 8 and 9 days post-infection. The samples were used for conventional paraffin section and H.E staining. We observed the early uninuclear schizonts and they were spherical in shape at 24-48 hour post-infection (hpi). Each schizonts contains 2-7merozoites at 72 hpi. Mature schizonts were seen at 96-120 h throughout the caecum and colon situated the gland epithelial cells and mature schizonts contain 10-20 merozites. Schizonts developed in the base of the glands of the caecum mature schizonts contain merozites at 144 hpi. Young gamonts were first observed in the glandular epithelium of the caecum and colon at 168 h after inoculation. According to the shape gametes, have two types existed in each of sexual generation, the microgametocyte and macrogamete. A microgametocyte contained lots of microgametes but macrogamete does not contain. Mature microgametes and macrogametes were sexual reproduction at 192 hpi. The gametes were development to oocysts and observed the oocysts in the glandular epithelium of the caecum and colon at 216 hpi. In the biological study, first, the unsporulated oocysts were treated with potassium dichromate and observed sporulation time and sporulation rate. The result showed the oocysts were sporulated at 18 h. The sporonts forward develop the cytoplasmic contraction. The sporoblast cytokinesis from one became to four and shrinkage triangle shaped at 26 h. Final stage, observed mature sporocyst at 32 h. In the second part of this study, the experiments were divided into 5 groups. In group A, as control that were not treated with ozone, but others in group B-E were treated with ozone for 30 mins, 60 mins, 90 mins and 120 min and then used for scanning electron micrographs (SEM) observed. The aforementioned 1,000 oocysts were then orally inoculated into each rabbits. The result showed the oocysts output in group A is more than group B-E. In SEM study, we observed oocysts surface structure seems to have been destroyed. The oocysts treated with ozone can reduce the oocysts output and destroyed the oocysts was observed. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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