Dsh-003- a pure compound extracted from Salvia multiorrhizae bunge inhibits Prostaglandin E2 induced survival and metastasis effects in HA22T Hepatocellular Carcinoma Cells
| Autor: | Yen-Hong Chou, 周彥宏 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Though the prognosis of hepatocellular Carcinoma (HCC) patients has been improved, their treatment options are limited and are often inefficient. Therefore, understanding the molecular mechanisms is critical for developing successful therapies to eradicate this disease. Earlier studies have shown that, Prostaglandin E2 (PGE2) enhances the growth of various cancers including breast, stomach, pancreas, lung and prostate by activating proteins that are involved in controlling proliferation and survival pathway. At first we found that Prostaglandin E Receptor 2 (EP2) and Prostaglandin E Receptor 4 (EP4) were overexpressed in liver cancer patients and in cell lines. Using cell line modes we found that PGE2 enhanced HA22T cell proliferation by inducing survival pathway marker such as p-EGFR, p-PI3K, p-Akt and their downstream anti-apoptotic proteins. Furthermore, PGE2 enhanced HA22T cell migration by inducing β-catenin expression and by decreasing E-cadherin and GSK3-β expression. We also found that PGE2 could not up-regulate MMP2 and MMP9 expression, however, PGE2 treatment increased EMT marker such as snail and vimentin in HA22T cells. All these results suggest us that, PGE2 enhanced HA22T cells survival and migration is by activating EP2 and EP4 receptor. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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