The antioxidant evaluation and active compoundsanalysis of supercritical fluid extracts of Pinusmorrisonicola Hay
| Autor: | Wen-Wing Li, 李文旺 |
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| Rok vydání: | 2013 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 101 Pinus morrisonicola Hay (PM), also known as “five leaves pine” is grown at 300-2300 m altitude in Taiwan. Traditionally, it has been used as beverages for lowing blood pressure. PM has been shown to reduce plasma cholesterol and LDL levels and to prevent hypertension. Supercritical fluid extraction (SCFE) technology has emerged as a superior alternative to the conventional techniques for extraction of natural products in food, pharmaceutical and chemical industries. SCFE technology is an environment, quick and safety extraction method. We used different conditions, temperature (40, 50, 60℃), pressure (15, 20, 25 MPa), time (5, 10, 15 min) and adjuvant solvents (1, 2, 3 ml ethanol) to extract PM and obtained PME 9 were obtained extracts or materials, respectively numbered 1 - 9, and the extraction yield were about 1.0~3.9 %. The aim of this study was to explore the antioxidant effects of PME and their active components. The cell viability of PME 1, 3, 4 and 6 had lower toxicity than that of PME 2, 5, 7, 8, 9. The results also shows that PME 3 (40℃、25 Mpa, 15 min and 3 ml ethanol) had the better extraction yield (3.9%) and potent antioxidant effects. The IC50 of scavenging DPPH radical is 20 mg/ml. The inhibition of Cu+2 (50 M)–induced oxidative LDL (100 g/ml) on TBARS and conjugated diene formation in the of PME 3 were 22% and 85% at 100 g/ml, respectively. In addition, PME 3 (100 g/ml) possessed effect and foam cells formation in Raw264.7 macrophage. The content of total polyphenol and flavonoids in PME3 was 14500 and 9600 g/g, and only gallic acid, epicatechin and p-coumaric acid were detected and confirmed by HPLC. Furthermore, we analyzed PME3 by GC/MS and found that the potent active compounds were Octadecane 1-(ethenyloxy) (55%), Neophytadiene (7.7%), Methyl abietate (3.4%) and 1-docosene (1.6%), respectively. Overall, PME3 has the potential for development as a antioxidant agent. Keywords：Pinus Morrisonicola Hay, Cardiovoscular disease, Supercritical fluid extraction |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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