Secondary Metabolites Isolation and Activity Assary on Fermented Legumes
| Autor: | Meng-Ju Chien, 簡孟儒 |
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| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 This study conducted biotransformation of various legume extracts (azuki beans, mung beans, soybeans, black beans, runner beans, and rice beans) using Aspergillus oryzae var.oryzae CCRC 31646 and Aspergillus oryzae var.oryzae IF0 5239, comparing influences of the DPPH free radical scavenging activity, tyrosinase activity inhibition, total flavonoid content, total phenolic content, E. coli inhibition, and P. acne inhibition before and after biotransformation. The results indicated that the DPPH free radical scavenging activity of the six legume extracts increased sharply after biotransformation. Of which, the scavenging rate of rice bean extracts was the highest at 97.65 % after the IFO 5239 biotransformation, an increase of 17.54 % compared to the rate before the biotransformation. The scavenging rate of azuki bean extracts increased 14.9 % to 97.53 % after the CCRC 31646 biotransformation. Excluding the rice bean extracts, the tyrosinase activities inhibition of the other five legumes increased by between 92.17 % and 313.68 % following the IFO 5239 biotransformation and by 60.63 % to 236.05 % following the CCRC 31646 biotransformation. After the biotransformation of IFO 5239 and CCRC 31646, the total flavonoid content of the azuki bean extracts rose by 85.94 % and 106.55 % respectively, and that of the soybean extracts increased 120.35 % and 132.89 %. The total phenolic content of the soybean extracts was 0.38 μg/ml after the IFO 5239 biotransformation, an increase of 40.74 %, and was 0.34 μg/ml after the CCRC 31646 biotransformation, an increase of 25.93 %. Before biotransformation and after the biotransformation of IFO 5239 and CCRC 31646, the E. coli antimicrobial activities of the mung bean extracts were 74 %, 92 %, and 100 %, and the antimicrobial activities of the rice bean extracts were 86 %, 97 %, and 93 %. The above results indicate that the antimicrobial activities of all the extracts improve significantly after biotransformation. In addition, the P. acne antimicrobial activities increased significantly after the biotransformations of IFO 5239 and CCRC 31646. The results of this study demonstrated the different capacities of legumes in various assays. Therefore, this study expects to apply the highly effective metabolites resulting from biotransformation to cosmetics and health food to increase their industry value. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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