Continuous Production of High-Content Isomalto-Oligosaccharides by Co-Immobilized Transglucosidase and Yeast Cell
| Autor: | Chun kai Yu, 游竣凱 |
|---|---|
| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 Through the catalytic reaction of transglucosidase (TG) on maltose, maltose is convented into panose, isomaltose and other isomalto-oligosaccharide (IMO) as well as glucose. During the reaction glucose is a by-product and is a competitive inhibitor against transglucosilaion activity of TG. Therefore, this IMO product consists not only of IMO (60% on a dry weight basis), but also large amounts of glucose and maltose. In this work, calcium alginate encapsulated TG and Pichia heimii served as biocatalysts. Via the catalytic reaction of TG, maltose is converted into IMO and glucose. And concurrently, glucose is consumed by cells of P. heimii. In this way, an IMO up to 76% of purity was obtained. This IMO syrup was successively fermented with immobilized “Fermipan” yeast and the purity of IMO increased to 94%. Compared with the reaction using TG alone, the current fermentation of yeast made the production of high purity IMO possible and more tetra- and penta-oligosaccharide to be produced. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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