PART I : Zebrafish as a model for safety assessments of D-serinePART II：Developmental expressions of two zebrafish ankh genes, ankha and ankhb
| Autor: | Xing-Guang Chen, 陳星光 |
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| Rok vydání: | 2012 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 100 PART I : Zebrafish as a model for safety assessments of D-serine The area where the nerve were known that synaptic terminals associate with muscle will rely on the calcium ion to transmit the signal, thus, at this area enriched lot of receptor,such as as NMDA receptor, AMPA receptor, kainate, helping the calcium ion transmission.With the permeability among them to calcium ion,the NMDA receptor has the highest permeability.The activation of the NMDA receptor needs to be dependent on some neurotransmitters,studied in the past, the mammal really exist the endogenous D-serine in the nervous system, this endogenous D-serine is one of the key factors for the activation of NMDA receptors In our study, zebrafish embryos by microinjection D-serine is indeed observed abnormal muscle fibers and neural differentiation, and showing the dose-dependent.To muscle,we found high concentrations of D-serine causes the fish appearance significant bending,muscle appearance serious irregular arrangement,and athletic ability is also greatly affected; in addition overexpression of D-serine also cause neuronal differentiation reduce, neural arrangement irregular than the wild species of zebra fish.Expression patterns are extremely similar caffeine,more further speculated that D-serine and calcium release of the indirect relationship. Because the important properties,this study will discuss the D-serine.Utilize through inject D-serine into zebrafish embryo, observe and analyse the D-serine toxicity for zebrafish embryo. PART II：Developmental expressions of two zebrafish ankh genes, ankha and ankhb Arthritic is a common condition affecting 3% of people aged 65–69 years,in which calcium containing crystals form in articular cartilage. The crystals most commonly involved consist of either calcium pyrophosphate dihydrate(CPPD) or calcium hydroxyapatite.Although the heritability of the condition in the general community is unknown,it have been described that loss of function of an inorganic pyrophosphate(PPi) transporter,the ANKH gene, has been demonstrated to cause excess calcium hydroxyapatite formation in the ank/ank mouse and this defect could be rescued by transfection of the wild-type ank into mutant fibroblasts.Zebrafish has two ANKH homologous genes,respectively ankha and ankhb.In this study whole-mount in situ hybridization wasutilized explore the two different gene signals in zebrafish early developmental stages. The experimental results show that both of ankha and ankhb are detected in 1 cell, 6 hpf, 12 hpf, 18 hpf of early embryos.In 24 hpf., 36 hpf., 48 hpf., 72 hpf. 5 dpf 7 dpf,the transcript is mainly concentrated in the head;The difference is that at 24 hpf and 36 hpf of ankhb will be manifested in the axial vein,intermediate cell mass and dorsal aorta.5 dpf,ankhb signal is weaker.7 dpf embryo ankha transcript was not detectable in 7 dpf. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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